e-ISSN 1828-1427
Rivista trimestrale di Sanità Pubblica Veterinaria edita dall'Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise ‘G. Caporale’
A quarterly journal devoted to veterinary public health, veterinary science and medicine published by the Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’ in Teramo, Italy
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ISSUES ONLINE
| 2015 - Volume 51 (4), October-December | |||
| Matthew Van der Saag, Adrian Nicholas, Michael Ward & Peter Kirkland | |||
| Evaluation of in vitro methods for assessment of infection of Australian Culicoides spp. with Bluetongue viruses | 335-343 | ||
| doi: 10.12834/VetIt.568.3144.3 | |||
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Summary Biting midges from the genus Culicoides (Diptera: Ceratopogonidae) are the vectors of several globally important arboviruses that affect livestock. These include orbiviruses from the Bluetongue virus (BTV) and African horse sickness virus (AHSV) groups and members of the Simbu serogroup of orthobunyaviruses, such as the recently emerged Schmallenberg virus. In this article, the authors evaluate several methods for feeding wild‑caught Australian Culicoides on BTV infected preparations of blood and sucrose. Feeding Culicoides on the membrane of embryonated chicken eggs was identified as the preferred feeding method. Although, cotton wool pads soaked in either virus‑infected blood or virus‑sucrose mixtures were also successful. A non‑destructive nucleic acid extraction technique for the detection of viral RNA in Culicoides was also evaluated as it allows for readily differentiating infected from non‑infected Culicoides. Keywords |
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