Index

e-ISSN 1828-1427

  

Rivista trimestrale di Sanità Pubblica Veterinaria edita dall'Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise ‘G. Caporale’

A quarterly journal devoted to veterinary public health, veterinary science and medicine published by the Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’ in Teramo, Italy

Editorial policy
Copyright and disclaimers
Guidelines for Authors
Editors
Honorary Members
Editorial Board
Scientific Advisory Board
Associate Editors
Issues online
Tariffs
Contact
 

ISSUES ONLINE

2015 - Volume 51 (4), October-December
   
 
Short communication
Prachi Sharma, David E. Stallknecht, Molly D. Murphy & Elizabeth W. Howerth		  
Expression of interleukin-1 beta and interleukin-6 in white-tailed deer infected with Epizootic haemorrhagic disease virus 283-288
doi: 10.12834/VetIt.556.2637.1
 
Summary
The pathogenesis of Epizootic haemorrhagic disease (EHD) in white‑tailed deer (Odocoileus virginianus, WTD) may be related to factors other than direct viral damage caused by replication in endothelium, such as the release of cytokines. This study focused on interleukin‑1 β (IL‑1) and interleukin‑6 (IL‑6), which have been shown to be variably upregulated in Bluetongue virus (BTV) infected cattle and sheep endothelial cultures possibly explaining species susceptibility to BTV. We evaluated circulating and tissue levels of IL‑1 and IL‑6 in WTD experimentally infected with EHD virus serotype 2 (EHDV‑2). Circulating levels of IL‑1 were assayed by ELISA. RT‑PCR and immunohistochemistry (IHC) were used to detect upregulation of IL‑1 and IL‑6 mRNA as well as protein expression, respectively. RT‑PCR was also used to determine whether IL‑1 and IL‑6 were upregulated in WTD peripheral blood mononuclear cells (PBMC) infected with EHDV‑2 in vitro. We found increased circulating levels of IL‑1 and upregulation of IL‑1 mRNA and protein expression and upregulation of IL‑6 mRNA in tissues of WTD infected with EHDV. Upregulation of mRNA levels of IL‑1 and IL‑6 in EHDV infected PBMCs was also observed. Findings suggest a role for IL‑1 and IL‑6 in the pathogenesis of EHD in WTD.

Keywords
Cytokine, Epizootic haemorrhagic disease viruses, Interleukin‑1 beta, Interleukin‑6, White‑tailed deer.


Full article

      
 
    © Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise 2015