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e-ISSN 1828-1427

  

Rivista trimestrale di Sanità Pubblica Veterinaria edita dall'Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise ‘G. Caporale’

A quarterly journal devoted to veterinary public health, veterinary science and medicine published by the Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’ in Teramo, Italy

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2009 - Volume 45 (2) April-June
   
 
Anakn De, Subhasis Batabyal, Sanchay K. Biswas, Karam Chand, Raj K. Singh & Bimalendu Mondal  
Surveillance of bluetongue virus antibody in goats using a recombinant VP7-based indirect ELISA in the coastal saline area of West Bengal, India 339-346
       

Summary
The authors describe the serological surveillance of bluetongue virus (BTV) group-specific antibody in goats of the coastal saline (Sunderban) area of West Bengal, India. A recombinant viral protein 7 (rVP7)-based indirect enzyme-linked immunosorbent assay (ELISA) was used to detect the antibody in sera. The bacterially expressed rVP7 was purified by affinity chromatography. The diagnostic performance of the assay was assessed by comparing it to the commercially available previously validated competitive ELISA. Using the control and 1 202 test sera, the cut-off value, sensitivity and specificity as well as other performance characteristics e.g. the Youden index, efficiency, positive and negative predictive value and prevalence were estimated. Field-collected goat sera (n = 1 202) were tested and a serological prevalence rate of 47% was observed in the study area.

Keywords
Bluetongue, Enzyme-linked immunosorbent assay, ELISA, Goat, India, Indirect enzyme-linked immunosorbent assay, Recombinant VP7, Serology, Surveillance, Virus.


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