Summary
A
reverse transcriptase real-time polymerase chain reaction was developed
to estimate the expression of the gamma-interferon (IFN- g) and
interleukin-4 (IL-4) in cattle vaccinated with Brucella abortus
RB51. Peripheral blood mononuclear cells of heifers vaccinated
with B. abortus RB51 were stimulated in vitro with
the same antigen and with Concanavalin A. The data obtained (presence
of IFN-g expression and absence of IL-4 expression) confirmed the
cell-mediated immune response to strain RB51 antigen. Furthermore,
the expression of these two cytokines was quantified and the results
showed values of IFN- g expression to be significantly higher in
vaccinated than non-vaccinated animals.
Keywords
Brucella
abortus,
Brucellosis, Cattle, Cell-mediated immunity, Cytokines, Interferons,
Reverse transcriptase polymerase chain reaction. |