Summary
Vero
cell adapted bluetongue virus (BTV), serotype 18 (BTV-18) was inactivated
with hydroxylamine and adjuvanted with aluminium hydroxide (Al[OH]3)
gel, saponin, or Al(OH)3 gel/saponin prior to immunisation
of sheep. Group-specific non-neutralising antibodies to BTV were
detected in all vaccinated sheep as soon as seven days after vaccination.
However, before challenge, serotype-specific neutralising antibodies
were not detected in vaccinated sheep. Upon challenge with homologous
live BTV, there was a reduction in delayed type of hypersensitivity
response in control sheep but not in the vaccinated animals. All
vaccinated sheep had significant (P<0.01) reductions in their
clinical reaction index (CRI) and duration of viraemia.
Keywords
Bluetongue
virus, Clinical reaction index, Delayed type of hypersensitivity,
Hydroxylamine, Immune response, Immunosuppression, Vaccines.
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