Summary
To
improve the efficiency and efficacy of cryopreservation of ovaries,
we developed a new method termed in situ oocyte (ISO) cryopreservation.
ISO cryopreservation is a multistep procedure that involves aspiration
of follicular fluid and then perfusion of antral follicles and diffusion
of whole buffalo ovaries with cryoprotectant agent (CPA), rapid
cooling, storage, thawing and, finally, dilution and removal of
the CPA with return to physiological environment. Our study compared
ISO cryo ovaries with cryo-diffused ovaries. We systematically examined
the effects of ISO cryo and diffuse cryo on ovaries by morphological
examination and with viability tests. The percentages of morphologically
normal and viable follicular oocytes from ISO cryo were significantly
higher than those that resulted from the cryo-diffused method (p<0.01).
The quality of follicular oocytes from ISO cryo ovaries appeared
better than that achieved from cryo-diffused ovaries. In conclusion,
this study shows that ISO cryo is highly efficient for cryopreservation
of oocytes and ovarian tissue.
Keywords
Buffalo,
Cryopreservation, Egypt, In situ, ISO, Oocyte, Ovary.
|