Summary
A
polyclonal antibody-based sandwich enzyme-linked immunosorbent assay
(s-ELISA)
was developed for the detection of bluetongue virus (BTV). The test
used antiserum against BTV and antiserum against the bluetongue
(BT) core protein. The antiserum against the virus was used as a
capture antibody and the antiserum against the protein was used
for detection. In this study, antiserum to recombinant viral protein
7 (rVP7) was used as a detection antibody in place of anti-core
antiserum. The assay was used to detect the BT serotypes found in
India, namely: 1, 2, 9, 15, 18 and 23. The modified sandwich assay
was able to detect BTV serotypes in cell culture supernatants. The
use of anti-rVP7 antiserum as the detection antibody avoids the
tedious and expensive purification of BTV core particles.
Keywords
Antiserum,
Anti-core antiserum, Anti-rVP7 antiserum, Bluetongue, ELISA, Enzyme-linked
immunosorbent assay, India, Sandwich ELISA, Virus. |