Summary
Salmonella
enterica serovars
Enteritidis and Typhimurium are the serotypes most frequently isolated
from human cases. Traditional surveillance systems, based on serological
characterisation and epidemiology, are not able to identify these
common strains that cause outbreaks in humans. Innovative techniques
are therefore necessary to accurately characterise these serotypes
and hence accelerate the identification of the primary sources.
Within a larger study, the goal of which was to develop an active
surveillance system for outbreaks of food-borne diseases, characterisation
of 42 Salmonella strains
was performed using molecular techniques (pulsed field gel electrophoresis
[PFGE] and random amplified polymorphic DNA [RAPD]), together with
the Kirby-Bauer antibiotic assay. Results showed that both techniques
were unable to satisfactorily characterise the Enteritidis serotype,
while only PFGE identified the Typhimurium serotype.
Keywords
Antibiotic
assays - Molecular typing - Pulsed-field gel electrophoresis - Random
amplified polymorphic DNA - Salmonella enterica Enteritidis
- Salmonella enterica Typhimurium, Salmonellosis.
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